goat anti fcgr2a Search Results


coralite488 conjugated goat anti rabbit igg h l  (Proteintech)
95
Proteintech coralite488 conjugated goat anti rabbit igg h l
Coralite488 Conjugated Goat Anti Rabbit Igg H L, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/goat+anti+fcgr2a/pmc11771321-163-13-23?v=Proteintech
Average 95 stars, based on 1 article reviews
coralite488 conjugated goat anti rabbit igg h l - by Bioz Stars, 2026-08
95/100 stars
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goat anti fcgr2a  (R&D Systems)
92
R&D Systems goat anti fcgr2a
Goat Anti Fcgr2a, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/goat+anti+fcgr2a/pmc06710049-134-49-53?v=R%26D+Systems
Average 92 stars, based on 1 article reviews
goat anti fcgr2a - by Bioz Stars, 2026-08
92/100 stars
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anti-lc3a  (MBL International)
90
MBL International anti-lc3a
Anti Lc3a, supplied by MBL International, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/goat+anti+fcgr2a/pmc05486365-363-125-127?v=MBL+International
Average 90 stars, based on 1 article reviews
anti-lc3a - by Bioz Stars, 2026-08
90/100 stars
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unconjugated anti-ap2  (Becton Dickinson)
90
Becton Dickinson unconjugated anti-ap2
Impaired CD1D1 internalization in Atg5-DC CKO DCs. CD1D1 surface levels are determined by the rates of CD1D1 internalization and recycling through endosomal and lysosomal compartments. EE, early endosomes; LE, late endosomes; Ly, lysosomes (A). Internalization of surface CD1D1 on splenic DCs or splenic B cells was analyzed using a biotin-based flow cytometric endocytosis assay. Pooled data and SEM of at least 3 independent experiments are shown. Each experiment contained at least 2 animals per group. Statistics: 2-tailed unpaired Student t test (B). CD1D1 recycling in splenic DCs or splenic B cells was analyzed using a flow cytometry based recycling assay. Pooled data and SEM of at least 3 independent experiments are shown. Each experiment contained at least 2 animals per group. Statistics: 2-tailed unpaired Student t test (C). Colocalization study of <t>AP2</t> and EEA1, as well as CD1D1 and EEA1 via confocal microscopy. Original magnification with 63×, 1.4 NA oil immersion lens. Representative photographs from 2 independent experiments per colocalization study are shown. Scale bar: 2.5 µm (D). Scatter dot plot representation and quantification of colocalization between AP2 and EEA1 and between CD1D1 and EEA1 via the Pearson coefficient. Each symbol represents one cell. Pooled data of 2 independent experiments per colocalization study are shown. Statistics: 2-tailed unpaired Student t test (E). *** P≤ 0.001,** P≤ 0.01,* ≤ 0.05, ns P> 0.05.
Unconjugated Anti Ap2, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/goat+anti+fcgr2a/pmc05486365-363-185-187?v=Becton+Dickinson
Average 90 stars, based on 1 article reviews
unconjugated anti-ap2 - by Bioz Stars, 2026-08
90/100 stars
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anti mouse cd40  (Bio X Cell)
96
Bio X Cell anti mouse cd40
Receptor-mediated uptake of glycolipid antigens and costimulatory properties remain unchanged in Atg5-DC CKO DCs. CD1D1-presentation of indicated iNKT cell agonists by primary splenic DCs was assessed using activation of the iNKT hybridoma A.407 as in Fig. 1B (A). Splenic DCs derived from either Atg5 floxed Ctrl or Atg5-DC CKO animals were stained for <t>CD40</t> surface expression. Pooled data and SEM of 3 independent experiments are shown. Each experiment contained at least 3 animals per group. Statistics: 2-tailed unpaired Student t test (B). Frequency of IL12B-producing splenic DCs upon CD40 ligation. Purified splenic DCs were either incubated with anti-CD40 overnight or left untreated. IL12B production was measured via intracellular cytokine staining and by ELISA. Results of 3 independent experiments are shown. Each experiment contained at least 3 animals per group. Statistics: 2-tailed unpaired Student t test (C). *** P≤ 0.001,** P≤ 0.01,* ≤ 0.05, ns P> 0.05. MFI, mean fluorescence intensity.
Anti Mouse Cd40, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/goat+anti+fcgr2a/pmc05486365-450-1-3?v=Bio+X+Cell
Average 96 stars, based on 1 article reviews
anti mouse cd40 - by Bioz Stars, 2026-08
96/100 stars
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expi 293 a iga southern biotech  (SouthernBiotech)
94
SouthernBiotech expi 293 a iga southern biotech
Receptor-mediated uptake of glycolipid antigens and costimulatory properties remain unchanged in Atg5-DC CKO DCs. CD1D1-presentation of indicated iNKT cell agonists by primary splenic DCs was assessed using activation of the iNKT hybridoma A.407 as in Fig. 1B (A). Splenic DCs derived from either Atg5 floxed Ctrl or Atg5-DC CKO animals were stained for <t>CD40</t> surface expression. Pooled data and SEM of 3 independent experiments are shown. Each experiment contained at least 3 animals per group. Statistics: 2-tailed unpaired Student t test (B). Frequency of IL12B-producing splenic DCs upon CD40 ligation. Purified splenic DCs were either incubated with anti-CD40 overnight or left untreated. IL12B production was measured via intracellular cytokine staining and by ELISA. Results of 3 independent experiments are shown. Each experiment contained at least 3 animals per group. Statistics: 2-tailed unpaired Student t test (C). *** P≤ 0.001,** P≤ 0.01,* ≤ 0.05, ns P> 0.05. MFI, mean fluorescence intensity.
Expi 293 A Iga Southern Biotech, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/goat+anti+fcgr2a/pmc07876222__DataSheet_1-83-62-65?v=SouthernBiotech
Average 94 stars, based on 1 article reviews
expi 293 a iga southern biotech - by Bioz Stars, 2026-08
94/100 stars
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fitc mp biomedicals  (Valiant Co Ltd)
96
Valiant Co Ltd fitc mp biomedicals
Receptor-mediated uptake of glycolipid antigens and costimulatory properties remain unchanged in Atg5-DC CKO DCs. CD1D1-presentation of indicated iNKT cell agonists by primary splenic DCs was assessed using activation of the iNKT hybridoma A.407 as in Fig. 1B (A). Splenic DCs derived from either Atg5 floxed Ctrl or Atg5-DC CKO animals were stained for <t>CD40</t> surface expression. Pooled data and SEM of 3 independent experiments are shown. Each experiment contained at least 3 animals per group. Statistics: 2-tailed unpaired Student t test (B). Frequency of IL12B-producing splenic DCs upon CD40 ligation. Purified splenic DCs were either incubated with anti-CD40 overnight or left untreated. IL12B production was measured via intracellular cytokine staining and by ELISA. Results of 3 independent experiments are shown. Each experiment contained at least 3 animals per group. Statistics: 2-tailed unpaired Student t test (C). *** P≤ 0.001,** P≤ 0.01,* ≤ 0.05, ns P> 0.05. MFI, mean fluorescence intensity.
Fitc Mp Biomedicals, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/goat+anti+fcgr2a/pm39213155-500-52-53?v=Valiant+Co+Ltd
Average 96 stars, based on 1 article reviews
fitc mp biomedicals - by Bioz Stars, 2026-08
96/100 stars
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anti mip-1b pe  (Becton Dickinson)
90
Becton Dickinson anti mip-1b pe
Receptor-mediated uptake of glycolipid antigens and costimulatory properties remain unchanged in Atg5-DC CKO DCs. CD1D1-presentation of indicated iNKT cell agonists by primary splenic DCs was assessed using activation of the iNKT hybridoma A.407 as in Fig. 1B (A). Splenic DCs derived from either Atg5 floxed Ctrl or Atg5-DC CKO animals were stained for <t>CD40</t> surface expression. Pooled data and SEM of 3 independent experiments are shown. Each experiment contained at least 3 animals per group. Statistics: 2-tailed unpaired Student t test (B). Frequency of IL12B-producing splenic DCs upon CD40 ligation. Purified splenic DCs were either incubated with anti-CD40 overnight or left untreated. IL12B production was measured via intracellular cytokine staining and by ELISA. Results of 3 independent experiments are shown. Each experiment contained at least 3 animals per group. Statistics: 2-tailed unpaired Student t test (C). *** P≤ 0.001,** P≤ 0.01,* ≤ 0.05, ns P> 0.05. MFI, mean fluorescence intensity.
Anti Mip 1b Pe, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/goat+anti+fcgr2a/pm39213155-500-33-35?v=Becton+Dickinson
Average 90 stars, based on 1 article reviews
anti mip-1b pe - by Bioz Stars, 2026-08
90/100 stars
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cl4051 chemicals  (Cedarlane)
96
Cedarlane cl4051 chemicals
Receptor-mediated uptake of glycolipid antigens and costimulatory properties remain unchanged in Atg5-DC CKO DCs. CD1D1-presentation of indicated iNKT cell agonists by primary splenic DCs was assessed using activation of the iNKT hybridoma A.407 as in Fig. 1B (A). Splenic DCs derived from either Atg5 floxed Ctrl or Atg5-DC CKO animals were stained for <t>CD40</t> surface expression. Pooled data and SEM of 3 independent experiments are shown. Each experiment contained at least 3 animals per group. Statistics: 2-tailed unpaired Student t test (B). Frequency of IL12B-producing splenic DCs upon CD40 ligation. Purified splenic DCs were either incubated with anti-CD40 overnight or left untreated. IL12B production was measured via intracellular cytokine staining and by ELISA. Results of 3 independent experiments are shown. Each experiment contained at least 3 animals per group. Statistics: 2-tailed unpaired Student t test (C). *** P≤ 0.001,** P≤ 0.01,* ≤ 0.05, ns P> 0.05. MFI, mean fluorescence intensity.
Cl4051 Chemicals, supplied by Cedarlane, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/goat+anti+fcgr2a/pm39213155-500-116-112?v=Cedarlane
Average 96 stars, based on 1 article reviews
cl4051 chemicals - by Bioz Stars, 2026-08
96/100 stars
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anti-cd107a pe-cy5  (Becton Dickinson)
90
Becton Dickinson anti-cd107a pe-cy5
Receptor-mediated uptake of glycolipid antigens and costimulatory properties remain unchanged in Atg5-DC CKO DCs. CD1D1-presentation of indicated iNKT cell agonists by primary splenic DCs was assessed using activation of the iNKT hybridoma A.407 as in Fig. 1B (A). Splenic DCs derived from either Atg5 floxed Ctrl or Atg5-DC CKO animals were stained for <t>CD40</t> surface expression. Pooled data and SEM of 3 independent experiments are shown. Each experiment contained at least 3 animals per group. Statistics: 2-tailed unpaired Student t test (B). Frequency of IL12B-producing splenic DCs upon CD40 ligation. Purified splenic DCs were either incubated with anti-CD40 overnight or left untreated. IL12B production was measured via intracellular cytokine staining and by ELISA. Results of 3 independent experiments are shown. Each experiment contained at least 3 animals per group. Statistics: 2-tailed unpaired Student t test (C). *** P≤ 0.001,** P≤ 0.01,* ≤ 0.05, ns P> 0.05. MFI, mean fluorescence intensity.
Anti Cd107a Pe Cy5, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/goat+anti+fcgr2a/pm39213155-500-2-8?v=Becton+Dickinson
Average 90 stars, based on 1 article reviews
anti-cd107a pe-cy5 - by Bioz Stars, 2026-08
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40592 v08h59 sars cov 2 omicron ba 1 rbd  (Sino Biological)
93
Sino Biological 40592 v08h59 sars cov 2 omicron ba 1 rbd
Receptor-mediated uptake of glycolipid antigens and costimulatory properties remain unchanged in Atg5-DC CKO DCs. CD1D1-presentation of indicated iNKT cell agonists by primary splenic DCs was assessed using activation of the iNKT hybridoma A.407 as in Fig. 1B (A). Splenic DCs derived from either Atg5 floxed Ctrl or Atg5-DC CKO animals were stained for <t>CD40</t> surface expression. Pooled data and SEM of 3 independent experiments are shown. Each experiment contained at least 3 animals per group. Statistics: 2-tailed unpaired Student t test (B). Frequency of IL12B-producing splenic DCs upon CD40 ligation. Purified splenic DCs were either incubated with anti-CD40 overnight or left untreated. IL12B production was measured via intracellular cytokine staining and by ELISA. Results of 3 independent experiments are shown. Each experiment contained at least 3 animals per group. Statistics: 2-tailed unpaired Student t test (C). *** P≤ 0.001,** P≤ 0.01,* ≤ 0.05, ns P> 0.05. MFI, mean fluorescence intensity.
40592 V08h59 Sars Cov 2 Omicron Ba 1 Rbd, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/goat+anti+fcgr2a/pm39213155-500-212-210?v=Sino+Biological
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40592 v08h59 sars cov 2 omicron ba 1 rbd - by Bioz Stars, 2026-08
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ebov gpdtm  (Mayflower Bioscience)
90
Mayflower Bioscience ebov gpdtm
Receptor-mediated uptake of glycolipid antigens and costimulatory properties remain unchanged in Atg5-DC CKO DCs. CD1D1-presentation of indicated iNKT cell agonists by primary splenic DCs was assessed using activation of the iNKT hybridoma A.407 as in Fig. 1B (A). Splenic DCs derived from either Atg5 floxed Ctrl or Atg5-DC CKO animals were stained for <t>CD40</t> surface expression. Pooled data and SEM of 3 independent experiments are shown. Each experiment contained at least 3 animals per group. Statistics: 2-tailed unpaired Student t test (B). Frequency of IL12B-producing splenic DCs upon CD40 ligation. Purified splenic DCs were either incubated with anti-CD40 overnight or left untreated. IL12B production was measured via intracellular cytokine staining and by ELISA. Results of 3 independent experiments are shown. Each experiment contained at least 3 animals per group. Statistics: 2-tailed unpaired Student t test (C). *** P≤ 0.001,** P≤ 0.01,* ≤ 0.05, ns P> 0.05. MFI, mean fluorescence intensity.
Ebov Gpdtm, supplied by Mayflower Bioscience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/goat+anti+fcgr2a/pmc09026287__mmc9-220-196-198?v=Mayflower+Bioscience
Average 90 stars, based on 1 article reviews
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Image Search Results


Impaired CD1D1 internalization in Atg5-DC CKO DCs. CD1D1 surface levels are determined by the rates of CD1D1 internalization and recycling through endosomal and lysosomal compartments. EE, early endosomes; LE, late endosomes; Ly, lysosomes (A). Internalization of surface CD1D1 on splenic DCs or splenic B cells was analyzed using a biotin-based flow cytometric endocytosis assay. Pooled data and SEM of at least 3 independent experiments are shown. Each experiment contained at least 2 animals per group. Statistics: 2-tailed unpaired Student t test (B). CD1D1 recycling in splenic DCs or splenic B cells was analyzed using a flow cytometry based recycling assay. Pooled data and SEM of at least 3 independent experiments are shown. Each experiment contained at least 2 animals per group. Statistics: 2-tailed unpaired Student t test (C). Colocalization study of AP2 and EEA1, as well as CD1D1 and EEA1 via confocal microscopy. Original magnification with 63×, 1.4 NA oil immersion lens. Representative photographs from 2 independent experiments per colocalization study are shown. Scale bar: 2.5 µm (D). Scatter dot plot representation and quantification of colocalization between AP2 and EEA1 and between CD1D1 and EEA1 via the Pearson coefficient. Each symbol represents one cell. Pooled data of 2 independent experiments per colocalization study are shown. Statistics: 2-tailed unpaired Student t test (E). *** P≤ 0.001,** P≤ 0.01,* ≤ 0.05, ns P> 0.05.

Journal: Autophagy

Article Title: The autophagy machinery restrains iNKT cell activation through CD1D1 internalization

doi: 10.1080/15548627.2017.1297907

Figure Lengend Snippet: Impaired CD1D1 internalization in Atg5-DC CKO DCs. CD1D1 surface levels are determined by the rates of CD1D1 internalization and recycling through endosomal and lysosomal compartments. EE, early endosomes; LE, late endosomes; Ly, lysosomes (A). Internalization of surface CD1D1 on splenic DCs or splenic B cells was analyzed using a biotin-based flow cytometric endocytosis assay. Pooled data and SEM of at least 3 independent experiments are shown. Each experiment contained at least 2 animals per group. Statistics: 2-tailed unpaired Student t test (B). CD1D1 recycling in splenic DCs or splenic B cells was analyzed using a flow cytometry based recycling assay. Pooled data and SEM of at least 3 independent experiments are shown. Each experiment contained at least 2 animals per group. Statistics: 2-tailed unpaired Student t test (C). Colocalization study of AP2 and EEA1, as well as CD1D1 and EEA1 via confocal microscopy. Original magnification with 63×, 1.4 NA oil immersion lens. Representative photographs from 2 independent experiments per colocalization study are shown. Scale bar: 2.5 µm (D). Scatter dot plot representation and quantification of colocalization between AP2 and EEA1 and between CD1D1 and EEA1 via the Pearson coefficient. Each symbol represents one cell. Pooled data of 2 independent experiments per colocalization study are shown. Statistics: 2-tailed unpaired Student t test (E). *** P≤ 0.001,** P≤ 0.01,* ≤ 0.05, ns P> 0.05.

Article Snippet: Antibodies, streptavidin, tetramer and iNKT lipid agonists Unconjugated anti-mouse CD40 (Bio X Cell, BE0016–2; clone: FGK4.5/ FGK45), unconjugated anti-mouse FCGR3A/CD16 and FCGR2A/CD32 (Bio X Cell, CUS-HB-197; clone 2.4G2), PE-conjugated anti-mouse CD1D1 (eBioscience, 12–0011–83; clone: 1B1), PE-conjugated rat IgG2bκ (eBioscience, 12–4031–83; clone: eB149/10H5), PE-conjugated anti-α-GalCer-CD1D1-complex (eBioscience, 12–2019–82; clone: L363), PE-conjugated anti-mouse IL12B (eBioscience, 12–7123–81; clone: C17.8), PerCP-Cy5.5-conjugated anti-mouse CD8A/CD8α (eBioscience, 45–0081–82; clone: 53.6.7), unconjugated anti-mouse CD1D1 (Biolegend, 123515; clone: 1B1), biotinylated anti-mouse CD1D1 (Biolegend, 123506; clone: 1B1), Pacific Blue-conjugated anti-mouse CD19 (Biolegend, 115523; clone: 6D5), Pacific Blue-conjugated anti-mouse CD4 (Biolegend, 100428; clone: GK1.5), PE-conjugated anti-mouse CD40 (Biolegend, 124610; clone: 3/23), PE-Cy7-conjugated anti-mouse ITGAX (Biolegend, 117318; clone: N418), APC-conjugated anti-mouse I-A b (clone: Biolegend, 107614; M5/114.15.2), APC-conjugated anti-mouse CD3E/CD3ε (Biolegend, 100236; clone: 17A2), PE-conjugated streptavidin (Biolegend, 405204), unconjugated anti-LC3A (MBL International Corporation, PM036), Alexa Fluor 488-conjugated F(ab')2 goat anti-rabbit IgG (H + L) (Thermo Fisher Scientific, A-11070) or -donkey anti-goat IgG (H + L) (Thermo Fisher Scientific, A-11055) and Alexa Fluor 555-conjugated F(ab')2 goat anti-rat (H + L) (Thermo Fisher Scientific, A-21434) or -rabbit anti-mouse IgG (H + L) (Thermo Fisher Scientific, A-21427), unconjugated anti-EEA1 (Antibodies-online, ABIN1439993), unconjugated anti-AP2 (BD Biosciences, 610502; clone 8/Adaptin α), PE-conjugated αGalCer-loaded CD1D1 tetramer (ProImmune, E001–2A), αGalCer (Adipogen, AG-CN2–0013-M001), GSL-1 and GalαGalCer were kindly provided by Paul B.

Techniques: Endocytosis Assay, Flow Cytometry, Confocal Microscopy

Receptor-mediated uptake of glycolipid antigens and costimulatory properties remain unchanged in Atg5-DC CKO DCs. CD1D1-presentation of indicated iNKT cell agonists by primary splenic DCs was assessed using activation of the iNKT hybridoma A.407 as in Fig. 1B (A). Splenic DCs derived from either Atg5 floxed Ctrl or Atg5-DC CKO animals were stained for CD40 surface expression. Pooled data and SEM of 3 independent experiments are shown. Each experiment contained at least 3 animals per group. Statistics: 2-tailed unpaired Student t test (B). Frequency of IL12B-producing splenic DCs upon CD40 ligation. Purified splenic DCs were either incubated with anti-CD40 overnight or left untreated. IL12B production was measured via intracellular cytokine staining and by ELISA. Results of 3 independent experiments are shown. Each experiment contained at least 3 animals per group. Statistics: 2-tailed unpaired Student t test (C). *** P≤ 0.001,** P≤ 0.01,* ≤ 0.05, ns P> 0.05. MFI, mean fluorescence intensity.

Journal: Autophagy

Article Title: The autophagy machinery restrains iNKT cell activation through CD1D1 internalization

doi: 10.1080/15548627.2017.1297907

Figure Lengend Snippet: Receptor-mediated uptake of glycolipid antigens and costimulatory properties remain unchanged in Atg5-DC CKO DCs. CD1D1-presentation of indicated iNKT cell agonists by primary splenic DCs was assessed using activation of the iNKT hybridoma A.407 as in Fig. 1B (A). Splenic DCs derived from either Atg5 floxed Ctrl or Atg5-DC CKO animals were stained for CD40 surface expression. Pooled data and SEM of 3 independent experiments are shown. Each experiment contained at least 3 animals per group. Statistics: 2-tailed unpaired Student t test (B). Frequency of IL12B-producing splenic DCs upon CD40 ligation. Purified splenic DCs were either incubated with anti-CD40 overnight or left untreated. IL12B production was measured via intracellular cytokine staining and by ELISA. Results of 3 independent experiments are shown. Each experiment contained at least 3 animals per group. Statistics: 2-tailed unpaired Student t test (C). *** P≤ 0.001,** P≤ 0.01,* ≤ 0.05, ns P> 0.05. MFI, mean fluorescence intensity.

Article Snippet: Unconjugated anti-mouse CD40 (Bio X Cell, BE0016–2; clone: FGK4.5/ FGK45), unconjugated anti-mouse FCGR3A/CD16 and FCGR2A/CD32 (Bio X Cell, CUS-HB-197; clone 2.4G2), PE-conjugated anti-mouse CD1D1 (eBioscience, 12–0011–83; clone: 1B1), PE-conjugated rat IgG2bκ (eBioscience, 12–4031–83; clone: eB149/10H5), PE-conjugated anti-α-GalCer-CD1D1-complex (eBioscience, 12–2019–82; clone: L363), PE-conjugated anti-mouse IL12B (eBioscience, 12–7123–81; clone: C17.8), PerCP-Cy5.5-conjugated anti-mouse CD8A/CD8α (eBioscience, 45–0081–82; clone: 53.6.7), unconjugated anti-mouse CD1D1 (Biolegend, 123515; clone: 1B1), biotinylated anti-mouse CD1D1 (Biolegend, 123506; clone: 1B1), Pacific Blue-conjugated anti-mouse CD19 (Biolegend, 115523; clone: 6D5), Pacific Blue-conjugated anti-mouse CD4 (Biolegend, 100428; clone: GK1.5), PE-conjugated anti-mouse CD40 (Biolegend, 124610; clone: 3/23), PE-Cy7-conjugated anti-mouse ITGAX (Biolegend, 117318; clone: N418), APC-conjugated anti-mouse I-A b (clone: Biolegend, 107614; M5/114.15.2), APC-conjugated anti-mouse CD3E/CD3ε (Biolegend, 100236; clone: 17A2), PE-conjugated streptavidin (Biolegend, 405204), unconjugated anti-LC3A (MBL International Corporation, PM036), Alexa Fluor 488-conjugated F(ab')2 goat anti-rabbit IgG (H + L) (Thermo Fisher Scientific, A-11070) or -donkey anti-goat IgG (H + L) (Thermo Fisher Scientific, A-11055) and Alexa Fluor 555-conjugated F(ab')2 goat anti-rat (H + L) (Thermo Fisher Scientific, A-21434) or -rabbit anti-mouse IgG (H + L) (Thermo Fisher Scientific, A-21427), unconjugated anti-EEA1 (Antibodies-online, ABIN1439993), unconjugated anti-AP2 (BD Biosciences, 610502; clone 8/Adaptin α), PE-conjugated αGalCer-loaded CD1D1 tetramer (ProImmune, E001–2A), αGalCer (Adipogen, AG-CN2–0013-M001), GSL-1 and GalαGalCer were kindly provided by Paul B.

Techniques: Activation Assay, Derivative Assay, Staining, Expressing, Ligation, Purification, Incubation, Enzyme-linked Immunosorbent Assay, Fluorescence

Autophagy proteins in DCs regulate iNKT cell responses to lipid immunization and microbial infection in vivo. Frequencies of iNKT cells (thymus, spleen and liver) from naïve Atg5 floxed Ctrl and Atg5-DC CKO mice were quantified. Representative dot plots from at least 2 independent experiments are shown (A). Serum concentration kinetics of IL4 and IFNG upon glycolipid challenge in vivo measured via ELISA (B). Surface expression kinetics of CD86 and CD40 on splenic DCs. Each symbol represents one animal. Pooled data of at least 2 independent experiments are shown. Statistics: 2-tailed unpaired Student t test (C). Quantification of bacterial titers (CFU/g tissue) in spleen and lung after in vivo infection of mice with S. paucimobilis. Each symbol represents one animal. One representative of 2 experiments with similar results is shown. Quantification of serum cytokines upon in vivo infection with S. paucimobilis. One representative of 2 experiments with similar results is shown. Statistics: 2-tailed Mann-Whitney U Test (D). *** P≤ 0.001,** P≤ 0.01,* ≤ 0.05, ns P> 0.05. MFI, mean fluorescence intensity.

Journal: Autophagy

Article Title: The autophagy machinery restrains iNKT cell activation through CD1D1 internalization

doi: 10.1080/15548627.2017.1297907

Figure Lengend Snippet: Autophagy proteins in DCs regulate iNKT cell responses to lipid immunization and microbial infection in vivo. Frequencies of iNKT cells (thymus, spleen and liver) from naïve Atg5 floxed Ctrl and Atg5-DC CKO mice were quantified. Representative dot plots from at least 2 independent experiments are shown (A). Serum concentration kinetics of IL4 and IFNG upon glycolipid challenge in vivo measured via ELISA (B). Surface expression kinetics of CD86 and CD40 on splenic DCs. Each symbol represents one animal. Pooled data of at least 2 independent experiments are shown. Statistics: 2-tailed unpaired Student t test (C). Quantification of bacterial titers (CFU/g tissue) in spleen and lung after in vivo infection of mice with S. paucimobilis. Each symbol represents one animal. One representative of 2 experiments with similar results is shown. Quantification of serum cytokines upon in vivo infection with S. paucimobilis. One representative of 2 experiments with similar results is shown. Statistics: 2-tailed Mann-Whitney U Test (D). *** P≤ 0.001,** P≤ 0.01,* ≤ 0.05, ns P> 0.05. MFI, mean fluorescence intensity.

Article Snippet: Unconjugated anti-mouse CD40 (Bio X Cell, BE0016–2; clone: FGK4.5/ FGK45), unconjugated anti-mouse FCGR3A/CD16 and FCGR2A/CD32 (Bio X Cell, CUS-HB-197; clone 2.4G2), PE-conjugated anti-mouse CD1D1 (eBioscience, 12–0011–83; clone: 1B1), PE-conjugated rat IgG2bκ (eBioscience, 12–4031–83; clone: eB149/10H5), PE-conjugated anti-α-GalCer-CD1D1-complex (eBioscience, 12–2019–82; clone: L363), PE-conjugated anti-mouse IL12B (eBioscience, 12–7123–81; clone: C17.8), PerCP-Cy5.5-conjugated anti-mouse CD8A/CD8α (eBioscience, 45–0081–82; clone: 53.6.7), unconjugated anti-mouse CD1D1 (Biolegend, 123515; clone: 1B1), biotinylated anti-mouse CD1D1 (Biolegend, 123506; clone: 1B1), Pacific Blue-conjugated anti-mouse CD19 (Biolegend, 115523; clone: 6D5), Pacific Blue-conjugated anti-mouse CD4 (Biolegend, 100428; clone: GK1.5), PE-conjugated anti-mouse CD40 (Biolegend, 124610; clone: 3/23), PE-Cy7-conjugated anti-mouse ITGAX (Biolegend, 117318; clone: N418), APC-conjugated anti-mouse I-A b (clone: Biolegend, 107614; M5/114.15.2), APC-conjugated anti-mouse CD3E/CD3ε (Biolegend, 100236; clone: 17A2), PE-conjugated streptavidin (Biolegend, 405204), unconjugated anti-LC3A (MBL International Corporation, PM036), Alexa Fluor 488-conjugated F(ab')2 goat anti-rabbit IgG (H + L) (Thermo Fisher Scientific, A-11070) or -donkey anti-goat IgG (H + L) (Thermo Fisher Scientific, A-11055) and Alexa Fluor 555-conjugated F(ab')2 goat anti-rat (H + L) (Thermo Fisher Scientific, A-21434) or -rabbit anti-mouse IgG (H + L) (Thermo Fisher Scientific, A-21427), unconjugated anti-EEA1 (Antibodies-online, ABIN1439993), unconjugated anti-AP2 (BD Biosciences, 610502; clone 8/Adaptin α), PE-conjugated αGalCer-loaded CD1D1 tetramer (ProImmune, E001–2A), αGalCer (Adipogen, AG-CN2–0013-M001), GSL-1 and GalαGalCer were kindly provided by Paul B.

Techniques: Infection, In Vivo, Concentration Assay, Enzyme-linked Immunosorbent Assay, Expressing, MANN-WHITNEY, Fluorescence